Journal: Journal of Cellular and Molecular Medicine

Article Title: A novel antisense lncRNA NT5E promotes progression by modulating the expression of SYNCRIP and predicts a poor prognosis in pancreatic cancer

doi: 10.1111/jcmm.15718

Figure Lengend Snippet: LncNT5E is up‐regulated in pancreatic cancer (PC) tissues and cell lines. A, The heat map from our previous lncRNA microarray reflected the differentially expressed lncRNAs in PC and normal tissues. T represents PC tissue, and N represents normal pancreatic tissue. ENST00000421594 indicates lncNT5E. B, Relative expression of lncNT5E in 45 paired PC and adjacent normal tissues. LncNT5E expression from all tissues was normalized to 18S expression (ΔCT) and then compared with adjacent normal tissues and converted to the fold change (2 −ΔΔCT ). C, Relative expression of lncNT5E in different cell lines. Data are shown as fold change (2 −ΔΔCT ). * P < .05, ** P < .01, *** P < .001

Article Snippet: We used microarray detection (H1602063, Arraystar Human LncRNA 8 × 60 k v3.0 1‐color) to study lncRNAs in three pairs of PC and adjacent normal tissues.

Techniques: Microarray, Expressing

Journal: Molecular Neurobiology

Article Title: Microarray Analysis of lncRNA and mRNA Expression Profiles in Patients with Neuromyelitis Optica

doi: 10.1007/s12035-016-9754-0

Figure Lengend Snippet: Validation of lncRNA microarray data by qRT-PCR. Three upregulated and three downregulated lncRNAs were validated by qRT-PCR of RNA extracted from PBMCs of 16 NMO patients and 16 healthy controls. The relative expression level of each lncRNA ( a upregulated lncRNAs, b downregulated lncRNAs) was normalized, and data displayed in histograms are expressed as means ± SD, ** P < 0.01 comparing NMO patients with healthy controls

Article Snippet: The Human IncRNA Microarray V3.0 (Arraystar, Rockville, MD, USA) was used to design the global profiling of human lncRNAs and protein-coding transcripts.

Techniques: Biomarker Discovery, Microarray, Quantitative RT-PCR, Expressing

Journal: Molecular Neurobiology

Article Title: Microarray Analysis of lncRNA and mRNA Expression Profiles in Patients with Neuromyelitis Optica

doi: 10.1007/s12035-016-9754-0

Figure Lengend Snippet: IncRNA-mRNA co-expression network. a Forty-three lncRNAs interacted with five mRNAs in the meaningful “NK-κB signaling pathway.” b Fifty-nine lncRNAs interacted with ten mRNAs in the GO of “cytokine-mediated signaling pathway.” c Fifty-eight lncRNAs interacted with nine mRNAs in the GO of “cytokine activity.” d Sixty-nine lncRNAs interacted with 14 mRNAs in the GO of “cellular response to cytokine stimulus”

Article Snippet: The Human IncRNA Microarray V3.0 (Arraystar, Rockville, MD, USA) was used to design the global profiling of human lncRNAs and protein-coding transcripts.

Techniques: Expressing, Activity Assay

Journal: Oncology Letters

Article Title: NAC1 promotes the migration of prostate cancer cells and participates in osteoclastogenesis by negatively regulating IFNβ

doi: 10.3892/ol.2017.7670

Figure Lengend Snippet: Profiling of NAC1-regulated genes in prostate cancer cells. (A) Hierarchical clustering of mRNA by the Z -score method. The results are displayed as a heat map, in which red indicates relatively high expression and green denotes relatively low expression. (B) Pathways corresponding to the up-regulated transcripts. (C) Pathways corresponding to down-regulated transcripts. (D) Western blots and RT-qPCR shown a significant reduction of NAC1 protein and mRNA in DU-145 cells transfected with NAC1 siRNAs compared with those transfected with NC (***P<0.001) (E). RT-qPCR was performed to validate the NAC1-regulated genes identified by microarray analysis in DU-145 cells transfected with siNAC1-1 or siNAC1-2 and NC. All candidate genes were validated (*P<0.05,**P<0.01,***P<0.001) by both siRNAs.

Article Snippet: An Arraystar Human messenger RNA (mRNA) Microarray v3.0 was used and run by the service provider.

Techniques: Expressing, Western Blot, Quantitative RT-PCR, Transfection, Microarray

Journal: Oncology Letters

Article Title: NAC1 promotes the migration of prostate cancer cells and participates in osteoclastogenesis by negatively regulating IFNβ

doi: 10.3892/ol.2017.7670

Figure Lengend Snippet: NAC1 promotes prostate cancer bone metastasis in a manner dependent on the induction of IFNβ. (A) NAC1 knockdown efficiency was verified in PC-3 cells by RT-qPCR and western blotting. (B) Genes associated with activation of osteoclasts were identified in RAW 264.7 cells by RT-qPCR and western blotting (**P<0.01, ***P<0.001). (C) The mRNA and protein levels of IFNβ were verified in PC-3 cells by RT-qPCR and western blotting (**P<0.01). (D) ELISA was performed to validate the protein level of IFNβ in the co-culture medium The differences between the siNAC1-1 or siNAC1-2 transfection groups and the NC group in the Figure were statistically significant (**P<0.01, ***P<0.001).

Article Snippet: An Arraystar Human messenger RNA (mRNA) Microarray v3.0 was used and run by the service provider.

Techniques: Knockdown, Quantitative RT-PCR, Western Blot, Activation Assay, Enzyme-linked Immunosorbent Assay, Co-Culture Assay, Transfection